Actin remodeling involves dynamic changes in the organization of actin filaments crucial for cell functions like motility, intracellular transport, and shape. Dysregulation of actin remodeling is associated with numerous diseases, including cancer and neurological disorders. This process can easily be investigated using TIRF microscopy, which allows for high-resolution imaging of near-surface cellular events, providing insights into the dynamic regulation of actin within cells.
The time-lapse above demonstrates actin remodeling observed in TIRF mode, using a 25x objective. The scale bar is set at 100 μm.
Comparing EPI vs. TIRF
The time-lapse above demonstrates actin remodeling observed in TIRF mode, utilizing a 60x objective. Scale bar 40 μm.